Gfp Lc3 Cell Line

Rfp acid stable and gfp acid sensitive.

Gfp lc3 cell line.

The raw264 7 cells stably expressing gfp lc3 white were incubated in complete media control and in earle s balanced salts solution starve or simultaneously supplemented with wortmannin starve wort for 1 h. To generate the cell line egfp lc3 rat plasmid was transfected into hek293a cells. Raw264 7 cells were transfected with pegfp lc3 plasmids. Single cell clones were selected and grown up.

Raw264 7 cell line stable expression of gfp lc3 is shown. The cells were selected with 800ug ml geneticin. These cells derived from the murine raw 264 7 macrophage cell line express the rfp gfp lc3 fusion protein where the murine lc3b microtubule associated protein 1 light chain 3 beta is fused to two fluorescent reporter proteins. The lc3b protein is a good marker to assess the autophagic flux since.

The cells were selected with 800ug ml geneticin. Also this cell line provides a useful tool for the study of autophagy using gfp lc3 as a standard assay read out by both immunofluorescence and biochemical methods. Also this cell line provides a useful tool for the study of autophagy using gfp lc3 as a standard assay read out by both immunofluorescence and biochemical methods. Production details egfp lc3 rat plasmid was transfected into hek293a cells.

So far many gfp lc3 expressed cell line from different species have been used to measure their autophagy activities including humans other mammals birds and fishes. This cell line uses gfp lc3 as a standard assay read out and can be evaluated by both immunofluorescence and biochemical methods. Single cell clones were selected and grown up. Single cell clones were selected and grown up.

However i can not get a very high transfection efficiency 10 and this expression disappears after 48hrs. To generate the cell line egfp lc3 rat plasmid was transfected into hek293a cells.