Gfp Stable Cell Line

4t1 gfp cmv stable cells express transgenically gfp under the control of the cmv promoter.

Gfp stable cell line.

In the studies of life science and biotechnology the commonly used reporter genes are visually identifiable including green fluorescent protein gfp red fluorescent protein rfp luciferase. Description transformed from sh sy5y cell line originated from a human bone marrow with neuroblastoma by lentivirus transduction this cell line express enhance egfp fluorescent marker. The green fluorescent protein gfp is a protein composed of 238 amino acid residues 26 9kda that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. 4t1 gfp cmv stable cells t4 a mouse mammary gland tumor cell line has been widely used in cancer research and drug development in vitro and in vivo.

I am trying to transfect thp 1 cells with gfp lc3 to create a stably expressing cell line. Gfp was expressed under the constitutive cmv promoter. However i can not get a very high transfection efficiency 10 and this expression disappears after 48hrs. Gfp reporter stable cell line hek293.

Stable cell line generation is made possible by the use of positive selection markers such as hygromycin g418 geneticin zeocin and blasticidin antibiotic resistance. Cell line contains a puromycin antibiotic selection marker.